Quantitative photoluminescence of broad band absorbing melanins: a procedure to correct for inner filter and re-absorption effects

TitleQuantitative photoluminescence of broad band absorbing melanins: a procedure to correct for inner filter and re-absorption effects
Publication TypeJournal Article
Year of Publication2005
AuthorsRiesz, J. J., Gilmore J. B., and Meredith Paul
JournalSpectrochimica Acta Part A-Molecular And Biomolecular Spectroscopy
Volume61
Issue9
Pagination2153–2160
AbstractWe report methods for correcting the photoluminescence emission and excitation spectra of highly absorbing samples for re-absorption and inner filter effects. We derive the general form of the correction, and investigate various methods for determining the parameters. Additionally, the correction methods are tested with highly absorbing fluorescein and melanin (broadband absorption) solutions; the expected linear relationships between absorption and emission are recovered upon application of the correction, indicating that the methods are valid. These procedures allow accurate quantitative analysis of the emission of low quantum yield samples (such as melanin) at concentrations where absorption is significant. (c) 2004 Elsevier B.V. All rights reserved.
DOI10.1016/j.saa.2004.08.014